Mihhail Kurašin "Processivity of cellulases and chitinases" | University of Tartu
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  • View all other units

Contacts of UT units

Faculty of Arts and Humanities
Faculty phone: 
(+372) 737 5341
Faculty address: 
Jakobi 2 - 116-121, 51014, Tartu
  • Institute of History and Archaeology
    Faculty phone: 
    (+372) 737 5651
    Faculty address: 
    Jakobi 2, 51003, Tartu
  • Institute of Estonian and General Linguistics
    Faculty phone: 
    (+372) 737 5221
    Faculty address: 
    Jakobi 2, 51014, Tartu
  • Institute of Philosophy and Semiotics
    Faculty phone: 
    (+372) 737 5314
    Faculty address: 
    Jakobi 2, III, rooms 309-352, 51014, Tartu
  • Institute of Cultural Research and Arts
    Faculty phone: 
    (+372) 737 5223
    Faculty address: 
    Ülikooli 16, 51003, Tartu
  • School of Theology and Religious Studies
    Faculty phone: 
    (+372) 737 5301
    Faculty address: 
    Ülikooli 18-310, 50090, Tartu
  • College of Foreign Languages and Cultures
    Faculty address: 
    Lossi 3, 51003, Tartu
  • Viljandi Culture Academy
    Faculty phone: 
    (+372) 435 5232
    Faculty address: 
    Posti 1, 71004, Viljandi
Faculty of Social Sciences
Faculty phone: 
(+372) 737 5957
Faculty address: 
Lossi 36, 51003, Tartu
  • Institute of Education
    Faculty phone: 
    (+372) 737 6440
    Faculty address: 
    Salme 1a, room 29, 50103, Tartu
  • Johan Skytte Institute of Political Studies
    Faculty phone: 
    (+372) 737 5582
    Faculty address: 
    Lossi 36, roomm 301, 51003, Tartu
  • School of Economics and Business Administration
    Faculty phone: 
    (+372) 737 6310
    Faculty address: 
    Narva mnt 4-A315, 51009, Tartu
  • Institute of Psychology
    Faculty phone: 
    (+372) 737 5902
    Faculty address: 
    Näituse 2, 50409, Tartu
  • School of Law
    Faculty phone: 
    (+372) 737 5390
    Faculty address: 
    Näituse 20, 50409, Tartu
  • Institute of Social Studies
    Faculty phone: 
    (+372) 737 5188
    Faculty address: 
    Lossi 36, 51003, Tartu
  • Narva College
    Faculty phone: 
    (+372) 740 1900
    Faculty address: 
    Raekoja plats 2, 20307, Narva
  • Pärnu College
    Faculty phone: 
    (+372) 445 0520
    Faculty address: 
    Ringi 35, 80012, Pärnu
Faculty of Medicine
Faculty phone: 
(+372) 737 5326
Faculty address: 
Ravila 19, 50411, Tartu
  • Institute of Biomedicine and Translational Medicine
    Faculty phone: 
    +(372) 737 4210
    Faculty address: 
    Biomeedikum, Ravila 19, 50411, Tartu
  • Institute of Pharmacy
    Faculty phone: 
    (+372) 737 5286
    Faculty address: 
    Nooruse 1, 50411, Tartu
  • Institute of Dentistry
    Faculty phone: 
    (+372) 731 9856
    Faculty address: 
    Raekoja plats 6, 51003, Tartu
  • Institute of Clinical Medicine
    Faculty phone: 
    (+372) 737 5323
    Faculty address: 
    L. Puusepa 8, 51014, Tartu, Eesti
  • Institute of Family Medicine and Public Health
    Faculty phone: 
    (+372) 737 4190
    Faculty address: 
    Ravila 19, 50411 Tartu
  • Institute of Sport Sciences and Physiotherapy
    Faculty phone: 
    (+372) 737 5360
    Faculty address: 
    Jakobi 5-205, 51014, Tartu
Faculty of Science and Technology
Faculty phone: 
(+372) 737 5820
Faculty address: 
Vanemuise 46-208, 51014, Tartu
  • Institute of Computer Science
    Faculty phone: 
    (+372) 737 5445
    Faculty address: 
    J. Liivi 2, 50409, Tartu
  • Estonian Marine Institute
    Faculty phone: 
    (+372) 671 8902
    Faculty address: 
    Mäealuse 14, 12618, Tallinn
  • Institute of Physics
    Faculty address: 
    W. Ostwaldi Str 1, 50411, Tartu
  • Institute of Chemistry
    Faculty phone: 
    (+372) 737 5261
    Faculty address: 
    Ravila 14a, 50411, Tartu
  • Institute of Mathematics and Statistics
    Faculty phone: 
    (+372) 737 5860
    Faculty address: 
    J. Liivi 2, 50409, Tartu
  • Institute of Molecular and Cell Biology
    Faculty phone: 
    (+372) 737 5011
    Faculty address: 
    Riia 23, 23b-134, 51010, Tartu
  • Institute of Technology
    Faculty phone: 
    (+372) 737 4800
    Faculty address: 
    Nooruse 1, 50411, Tartu
  • Institute of Ecology and Earth Sciences
    Faculty phone: 
    (+372) 737 5835
    Faculty address: 
    Vanemuise 46, 51014, Tartu
Institutions
  • Library
    Faculty phone: 
    (+372) 737 5702
    Faculty address: 
    W.Struve 1, 50091, Tartu
  • Youth Academy
    Faculty phone: 
    (+372) 737 5581
    Faculty address: 
    Lossi 38, 51003, Tartu
  • Estonian Genome Center
    Faculty phone: 
    (+372) 737 4000
    Faculty address: 
    Riia 23b, 51010, Tartu
  • Museum
    Faculty phone: 
    (+372) 737 5674
    Faculty address: 
    Lossi 25, 51014, Tartu
  • University of Tartu Natural History Museum and Botanical Garden
    Faculty phone: 
    (+372) 737 6076
    Faculty address: 
    Vanemuise 46, 51014, Tartu
Support Units
  • Administrative Office
    Faculty phone: 
    (+372) 737 5606
    Faculty address: 
    Ülikooli 18a, 51014, Tartu
  • University Office in Tallinn
    Faculty phone: 
    +372 737 6600
    Faculty address: 
    Teatri väljak 3, 10143 Tallinn
  • Estates Office
    Faculty phone: 
    (+372) 737 5137
    Faculty address: 
    Ülikooli 18a, 51014, Tartu
  • Finance Office
    Faculty phone: 
    (+372) 737 5125
    Faculty address: 
    Jakobi 4, 51014, Tartu
  • Information Technology Office
    Faculty phone: 
    (+372) 737 6000, arvutiabi: 737 5500
    Faculty address: 
    Ülikooli 18a, 51014, Tartu
  • Internal Audit Office
    Faculty address: 
    Ülikooli 17-114, 51014, Tartu
  • Marketing and Communication Office
    Faculty phone: 
    (+372) 737 5687
    Faculty address: 
    Ülikooli 18, ruum 210, 50090, Tartu
  • Office of Academic Affairs
    Faculty phone: 
    (+372) 737 6215
    Faculty address: 
    Ülikooli 18, 50090, Tartu
  • Office of Research and Development
    Faculty phone: 
    (+372) 737 6192
    Faculty address: 
    Lossi 3, III korrus, 51003, Tartu
  • Human Resources Office
    Faculty phone: 
    (+372) 737 5145
    Faculty address: 
    Ülikooli 18, rooms 302 and 304, 50090, Tartu
  • Rector's Strategy Office
    Faculty phone: 
    (+372) 737 5600
    Faculty address: 
    Ülikooli 18, 51014, Tartu
  • Student Council
    Faculty phone: 
    (+372) 737 5400
    Faculty address: 
    Ülikooli 18b, 51014, Tartu
Other Units
  • University of Tartu Academic Sports Club
    Faculty phone: 
    +372 737 5371
    Faculty address: 
    Ujula 4, 51008 Tartu
  • Tartu Student Village
    Faculty phone: 
    +372 740 9959
    Faculty address: 
    Narva mnt 25, 51013 Tartu
  • Tartu Students’ Club
    Faculty phone: 
    +372 730 2400
    Faculty address: 
    Kalevi 24, 51010 Tartu
  • University of Tartu Press
    Faculty phone: 
    +372 737 5945
    Faculty address: 
    W. Struve 1, 50091 Tartu
  • Tartu University Hospital
    Faculty address: 
    L. Puusepa 1a, 50406 Tartu
  • University of Tartu Foundation
    Faculty phone: 
    +372 737 5852
    Faculty address: 
    Ülikooli 18, 50090 Tartu
  • View all other units

Mihhail Kurašin "Processivity of cellulases and chitinases"

Date: 
24.03.2017 - 10:15

Mihhail Kurašin will defend his doctoral thesis titled „Processivity of cellulases and chitinases” on 24 March 2017 at 10:15.

Supervisor:
Senior Research Fellow Priit Väljamäe, University of Tartu

Opponent:
Associate Professor Jerry Ståhlberg, Sveriges Lantbruksuniversitet (SLU), Uppsala, Sweden

Summary: 
Dwindling oil reserves and increasing world population necessitate the search for alternative sources of energy. Thus, cellulose and chitin, the net annual production of which are estimated in billions of tons, have recently become potential candidates as a renewable sources for the production of biofuel for gasoline working vehicles as well as essential chemical compounds for food and pharmaceutical industries. The main idea lies in degradation of cellulose and chitin into monomers (i.e. glucose and N- acetylglucosamine respectively). The latter can be easily converted into ethanol and other chemicals. In nature cellulose is degraded by enzymes with common name cellulases and chitin is degraded by enzymes called chitinases. Cellulases and chitinases are also employed for industrial uses since they enable to avoid the use of high temperatures and concentrated mineral acids. However, natural enzymes are slow and their contribution to the cost of the end products remains high. Efficient degradation of polysaccharides is achieved by concerted action of different types of enzymes. The main contribution to this process generally comes from processive enzymes that constitute the major part of cellulolytic/chitinolytic systems. Processivity is defined as the ability of an enzyme to conduct sequential catalytic acts without dissociating from its substrate. It is therefore an important characteristic of cellulose/chitin degrading enzymes, and its investigation is likely to provide a good clue to the improvement of the process of saccharification of recalcitrant polysaccharides. The present study was aimed at biochemical description of processive cellulases and chitinases. New methods for the measurement of processivity and other kinetic parameters were developed. It was previously know that the limiting step in the catalytic cycle of enzyme acting in isolation is the dissociation from the substrate. On the contrary we found that the limiting step in the catalytic cycle of enzyme acting in the synergistic mixture is the processive movement along the polymer chain. We also demonstrated that for the efficient degradation of recalcitrant polysaccharides strong interactions in the substrate binding site and in the product binding site are required. The loss of one interaction leads to reduced activity on crystalline substrates but concomitantly increases activity on amorphous substrates.

Location: 
Riia 23b-105
Event category: