Ganesh Babu Manoharan "Combining chemical and genetic approaches for photoluminescence assays of protein kinases" | University of Tartu

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Contacts of UT units

Faculty of Arts and Humanities
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Faculty address: 
Jakobi 2, rooms 116–121, 51005 Tartu
  • Dean's Office
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    Jakobi 2, rooms 116–121, 51005 Tartu
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    Jakobi 2, 51005 Tartu
  • Institute of Estonian and General Linguistics
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  • Institute of Philosophy and Semiotics
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    Jakobi 2, rooms 309–352, 51005 Tartu
  • Institute of Cultural Research
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    Ülikooli 16, 51003 Tartu
  • School of Theology and Religious Studies
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    Ülikooli 18–310, 50090 Tartu
  • College of Foreign Languages and Cultures
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  • Viljandi Culture Academy
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Lossi 36, 51003 Tartu
  • Dean's Office
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  • Institute of Education
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  • Johan Skytte Institute of Political Studies
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  • School of Economics and Business Administration
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  • Institute of Physics
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  • Youth Academy
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    Uppsala 10, 51003 Tartu
  • Institute of Genomics
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    Faculty address: 
    Riia 23b, 51010 Tartu
  • Museum
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    +372 737 5674
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    Lossi 25, 51003 Tartu
  • University of Tartu Natural History Museum and Botanical Garden
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    Faculty address: 
    Vanemuise 46, 51003 Tartu
Support Units
  • Administrative Office
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    Ülikooli 18a, 51005 Tartu
  • Centre for Entrepreneurship and Innovation
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    +372 737 6339
    Faculty address: 
    Lossi 3, III floor, 51003 Tartu
  • University Office in Tallinn
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    +372 737 6600
    Faculty address: 
    Teatri väljak 3, 10143 Tallinn
  • Estates Office
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    +372 737 5137
    Faculty address: 
    Ülikooli 18a, 51005 Tartu
  • Finance Office
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    +372 737 5125
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    Jakobi 4, 51005 Tartu
  • Grant Office
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    Lossi 3, III floor, 51003 Tartu
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    Ülikooli 18a, 51005 Tartu
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  • Marketing and Communication Office
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    Ülikooli 18a, 51005 Tartu
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  • University of Tartu Press
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  • View all other units

Ganesh Babu Manoharan "Combining chemical and genetic approaches for photoluminescence assays of protein kinases"

Date: 
29.03.2016 - 10:00

Ganesh Babu Manoharan will defend his doctoral thesis titled "Combining chemical and genetic approaches for photoluminescence assays of protein kinases" on 29 March at 10:00 at UT Chemicum (Ravila Str. 14a, room 1021).

Supervisor: Asko Uri (PhD), TÜ Keemia Instituut

Opponent: Ruslan Dmitriev (PhD), University College Cork, Ireland

Summary: In this study, joint application of chemical and genetic approaches was used for construction of sensor systems for analysis of protein kinases.

Phosphorylation reaction is an important post-translational protein modification procedure in cells, which is carried out by protein kinases (PKs). This modification leads to increase in the diversity of the proteome that influences various aspects of normal and pathological physiology. Dysregulation of protein phosphorylation balances, caused by the aberrant activity of PKs is a cause or consequence of several complex diseases such as cancers, inflammatory disorders, cardio-vascular diseases and diabetes. Therefore PKs have become important drug targets in the 21st century. In recent 15 years, 30 small-molecule PK inhibitors have been approved for use in clinical practice.

In addition to being a potential drug targets, PKs also serve as biomarkers for cancers and other diseases, as altered expression level of various PKs is observed in a variety of malignancies. Thus there is high demand for analytical methods that enable determination of expression and activity levels of specific PKs in clinical samples, such as bodily fluids and cancerous tissues. Thus high throughput assays for screening PK inhibitors also form an important component of drug development pipeline.

During the last decade, it has been understood that merging of two disciplines, synthetic chemistry and protein engineering, is needed to construct molecules and their complexes with new functionalities that can create novel opportunities for biomedical research, drug development and disease diagnostics.

In this thesis combination of chemical and genetic approaches was used for the development of analytical tools for the characterization of PKs and PK inhibitors. PKs (PKAc, CK2α, and PIM kinases) were fused with fluorescent proteins (FPs). On the other hand, ARC-based small-molecule PK inhibitors were developed into protein binding-responsive ARC-Lum probes possessing unique photoluminescent properties. Joint application of PKs fused with fluorescent proteins and ARC-Lum probes enabled the construction of sensor systems that can be used for specific and sensitive determination of PKs in biological samples and as research tools for mapping and monitoring PK activity in living cells.

Organizer: 
Säde Viirlaid
Location: 
Ravila Street 14a, room 1021
Event category: