Jaanus Liigand, „Standard substance free quantification for LC/ESI/MS analysis based on the predicted ionization efficiencies"

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View all other units

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Date:

22.08.2019 - 13:15

On 22. August 2019 at 13.15 Jaanus Liigand will defend his doctoral thesis ”Standard substance free quantification for LC/ESI/MS analysis based on the predicted ionization efficiencies”.

Supervisor:
senior Research Fellow, Anneli Kruve-Viil, UT

Opponent:
professor Jonathan Martin, University of Stockholmi, Sweden

Summary:
Liquid chromatography electrospray ionization mass spectrometry (LC/ESI/MS) is a widely applied analytical technique. Furthermore, it is more and more applied in non-target analysis enabling to obtain a more complete picture of biological and chemical processes occurring in living organisms and environment. Standard substances are needed to obtain quantitative information from LC/ESI/MS analysis.
The ultimate aim of my doctoral thesis is to provide a solution to overcome the need of standard substances and to quantify compounds detected and identified with LC/ESI/MS. I propose an approach of estimating the concentration of detected and identified compounds in LC/ESI/MS analysis using predicted electrospray ionization efficiencies and using the ionization efficiency to convert LC/ESI/MS signals to the concentrations of compounds.
To achieve this, I studied the influence of compound structure to ionization efficiency on a large set of compounds. Furthermore, I studied the effect of chromatographic eluent to ionization efficiency covering most widely used organic modifiers, additives and pH. Thirdly, the effect of instrumentation on ionization efficiency was studied and finally the model was developed. Moreover, I developed an approach named shortly Quantem to provide standard substance free quantification in LC/ESI/MS analysis that accounts for specific sample (analytes and matrix) and specific method (eluents used, gradient program and instrumentation). The developed Quantem approach is made available as a software to LC/ESI/MS community.

Location:

Ravila 14A-1020

URL:

hdl.handle.net/10062/64819

Event category:

Defence

M	T	W	T	F	S	S
			1 Matti Vainio's exhibition "Findings \| Leiud" The Exhibition "The enchanting art of stone cutting. Morgenstern 250" Estonia's National University 100: Photo exhibition “Put on Your Thinking Cap! The Hundred Faces of the University of Tartu” Aarne Maasik’s Photos of Tartu Cathedral	2 Egon Urgard "Potential therapeutic approaches for modulation of inflammatory response pathways" Matti Vainio's exhibition "Findings \| Leiud" The Exhibition "The enchanting art of stone cutting. Morgenstern 250" Estonia's National University 100: Photo exhibition “Put on Your Thinking Cap! The Hundred Faces of the University of Tartu” Aarne Maasik’s Photos of Tartu Cathedral	3 Matti Vainio's exhibition "Findings \| Leiud" The Exhibition "The enchanting art of stone cutting. Morgenstern 250" Estonia's National University 100: Photo exhibition “Put on Your Thinking Cap! The Hundred Faces of the University of Tartu” Aarne Maasik’s Photos of Tartu Cathedral	4 Matti Vainio's exhibition "Findings \| Leiud" The Exhibition "The enchanting art of stone cutting. Morgenstern 250" Estonia's National University 100: Photo exhibition “Put on Your Thinking Cap! The Hundred Faces of the University of Tartu” Aarne Maasik’s Photos of Tartu Cathedral
5 Indrek Teino “Studies on aryl hydrocarbon receptor in the mouse granulosa cell model” Matti Vainio's exhibition "Findings \| Leiud" The Exhibition "The enchanting art of stone cutting. Morgenstern 250" Estonia's National University 100: Photo exhibition “Put on Your Thinking Cap! The Hundred Faces of the University of Tartu” Aarne Maasik’s Photos of Tartu Cathedral	6 Welcome Reception for International Staff and PhD Students Matti Vainio's exhibition "Findings \| Leiud" The Exhibition "The enchanting art of stone cutting. Morgenstern 250" Estonia's National University 100: Photo exhibition “Put on Your Thinking Cap! The Hundred Faces of the University of Tartu” Aarne Maasik’s Photos of Tartu Cathedral	7 Matti Vainio's exhibition "Findings \| Leiud" The Exhibition "The enchanting art of stone cutting. Morgenstern 250" Estonia's National University 100: Photo exhibition “Put on Your Thinking Cap! The Hundred Faces of the University of Tartu” Aarne Maasik’s Photos of Tartu Cathedral	8 Matti Vainio's exhibition "Findings \| Leiud" The Exhibition "The enchanting art of stone cutting. Morgenstern 250" Estonia's National University 100: Photo exhibition “Put on Your Thinking Cap! The Hundred Faces of the University of Tartu” Aarne Maasik’s Photos of Tartu Cathedral	9 Seminar focusing on SARS-CoV-2/COVID19 research Matti Vainio's exhibition "Findings \| Leiud" The Exhibition "The enchanting art of stone cutting. Morgenstern 250" Estonia's National University 100: Photo exhibition “Put on Your Thinking Cap! The Hundred Faces of the University of Tartu” Aarne Maasik’s Photos of Tartu Cathedral	10 Matti Vainio's exhibition "Findings \| Leiud" The Exhibition "The enchanting art of stone cutting. Morgenstern 250" Estonia's National University 100: Photo exhibition “Put on Your Thinking Cap! The Hundred Faces of the University of Tartu” Aarne Maasik’s Photos of Tartu Cathedral	11 Matti Vainio's exhibition "Findings \| Leiud" The Exhibition "The enchanting art of stone cutting. Morgenstern 250" Estonia's National University 100: Photo exhibition “Put on Your Thinking Cap! The Hundred Faces of the University of Tartu” Aarne Maasik’s Photos of Tartu Cathedral
12 Matti Vainio's exhibition "Findings \| Leiud" The Exhibition "The enchanting art of stone cutting. Morgenstern 250" Estonia's National University 100: Photo exhibition “Put on Your Thinking Cap! The Hundred Faces of the University of Tartu” Aarne Maasik’s Photos of Tartu Cathedral	13 Sofia Raquel Alves Oliveira “HPLC analysis of bacterial alarmone nucleotide (p)ppGpp and its toxic analogue ppApp” Birgit Viru “Snow cover dynamics and its impact on greenhouse gas fluxes in drained peatlands in Estonia” Kati Braschinsky “Epidemiology of primary headaches in Estonia and applicability of web-based solutions in headache epidemiology research” Ardi Tampuu “Neural Networks for Analyzing Biological Data” Iuliia Burdun "Improving groundwater table monitoring for Northern Hemisphere peatlands using optical and thermal satellite data" Matti Vainio's exhibition "Findings \| Leiud" The Exhibition "The enchanting art of stone cutting. Morgenstern 250" Estonia's National University 100: Photo exhibition “Put on Your Thinking Cap! The Hundred Faces of the University of Tartu” Aarne Maasik’s Photos of Tartu Cathedral	14 Matti Vainio's exhibition "Findings \| Leiud" The Exhibition "The enchanting art of stone cutting. Morgenstern 250" Estonia's National University 100: Photo exhibition “Put on Your Thinking Cap! The Hundred Faces of the University of Tartu” Aarne Maasik’s Photos of Tartu Cathedral	15 Events dedicated to the anniversary of the Faculty of Medicine 2020 Matti Vainio's exhibition "Findings \| Leiud" The Exhibition "The enchanting art of stone cutting. Morgenstern 250" Estonia's National University 100: Photo exhibition “Put on Your Thinking Cap! The Hundred Faces of the University of Tartu” Aarne Maasik’s Photos of Tartu Cathedral	16 Events dedicated to the anniversary of the Faculty of Medicine 2020 Matti Vainio's exhibition "Findings \| Leiud" The Exhibition "The enchanting art of stone cutting. Morgenstern 250" Estonia's National University 100: Photo exhibition “Put on Your Thinking Cap! The Hundred Faces of the University of Tartu” Aarne Maasik’s Photos of Tartu Cathedral	17 Matti Vainio's exhibition "Findings \| Leiud" The Exhibition "The enchanting art of stone cutting. Morgenstern 250" Estonia's National University 100: Photo exhibition “Put on Your Thinking Cap! The Hundred Faces of the University of Tartu” Aarne Maasik’s Photos of Tartu Cathedral	18 Matti Vainio's exhibition "Findings \| Leiud" The Exhibition "The enchanting art of stone cutting. Morgenstern 250" Estonia's National University 100: Photo exhibition “Put on Your Thinking Cap! The Hundred Faces of the University of Tartu” Aarne Maasik’s Photos of Tartu Cathedral
19 Matti Vainio's exhibition "Findings \| Leiud" The Exhibition "The enchanting art of stone cutting. Morgenstern 250" Estonia's National University 100: Photo exhibition “Put on Your Thinking Cap! The Hundred Faces of the University of Tartu” Aarne Maasik’s Photos of Tartu Cathedral	20 Matti Vainio's exhibition "Findings \| Leiud" The Exhibition "The enchanting art of stone cutting. Morgenstern 250" Estonia's National University 100: Photo exhibition “Put on Your Thinking Cap! The Hundred Faces of the University of Tartu” Aarne Maasik’s Photos of Tartu Cathedral	21 Matti Vainio's exhibition "Findings \| Leiud" The Exhibition "The enchanting art of stone cutting. Morgenstern 250" Estonia's National University 100: Photo exhibition “Put on Your Thinking Cap! The Hundred Faces of the University of Tartu” Aarne Maasik’s Photos of Tartu Cathedral	22 Dorpater Dozentenabend international faculty club season’s opening meeting Matti Vainio's exhibition "Findings \| Leiud" The Exhibition "The enchanting art of stone cutting. Morgenstern 250" Estonia's National University 100: Photo exhibition “Put on Your Thinking Cap! The Hundred Faces of the University of Tartu” Aarne Maasik’s Photos of Tartu Cathedral	23 Matti Vainio's exhibition "Findings \| Leiud" The Exhibition "The enchanting art of stone cutting. Morgenstern 250" Estonia's National University 100: Photo exhibition “Put on Your Thinking Cap! The Hundred Faces of the University of Tartu” Aarne Maasik’s Photos of Tartu Cathedral	24 Planetarium show (in Russian) at the Tartu Old Observatory Matti Vainio's exhibition "Findings \| Leiud" The Exhibition "The enchanting art of stone cutting. Morgenstern 250" Estonia's National University 100: Photo exhibition “Put on Your Thinking Cap! The Hundred Faces of the University of Tartu” Aarne Maasik’s Photos of Tartu Cathedral	25 Matti Vainio's exhibition "Findings \| Leiud" The Exhibition "The enchanting art of stone cutting. Morgenstern 250" Estonia's National University 100: Photo exhibition “Put on Your Thinking Cap! The Hundred Faces of the University of Tartu” Aarne Maasik’s Photos of Tartu Cathedral
26 Matti Vainio's exhibition "Findings \| Leiud" The Exhibition "The enchanting art of stone cutting. Morgenstern 250" Estonia's National University 100: Photo exhibition “Put on Your Thinking Cap! The Hundred Faces of the University of Tartu” Aarne Maasik’s Photos of Tartu Cathedral	27 Matti Vainio's exhibition "Findings \| Leiud" The Exhibition "The enchanting art of stone cutting. Morgenstern 250" Estonia's National University 100: Photo exhibition “Put on Your Thinking Cap! The Hundred Faces of the University of Tartu” Aarne Maasik’s Photos of Tartu Cathedral	28 Triinu Vihmann “Ionic liquids: synthesis and application in lubrication and lithography” Matti Vainio's exhibition "Findings \| Leiud" The Exhibition "The enchanting art of stone cutting. Morgenstern 250" Estonia's National University 100: Photo exhibition “Put on Your Thinking Cap! The Hundred Faces of the University of Tartu” Aarne Maasik’s Photos of Tartu Cathedral	29 Matti Vainio's exhibition "Findings \| Leiud" The Exhibition "The enchanting art of stone cutting. Morgenstern 250" Estonia's National University 100: Photo exhibition “Put on Your Thinking Cap! The Hundred Faces of the University of Tartu” Aarne Maasik’s Photos of Tartu Cathedral	30 Matti Vainio's exhibition "Findings \| Leiud" The Exhibition "The enchanting art of stone cutting. Morgenstern 250" Estonia's National University 100: Photo exhibition “Put on Your Thinking Cap! The Hundred Faces of the University of Tartu” Aarne Maasik’s Photos of Tartu Cathedral	31 Matti Vainio's exhibition "Findings \| Leiud" The Exhibition "The enchanting art of stone cutting. Morgenstern 250" Estonia's National University 100: Photo exhibition “Put on Your Thinking Cap! The Hundred Faces of the University of Tartu” Aarne Maasik’s Photos of Tartu Cathedral

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Jaanus Liigand, „Standard substance free quantification for LC/ESI/MS analysis based on the predicted ionization efficiencies"

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