Margus Leppik "Substrate specificity of the multisite specific pseudouridine synthase RluD" | University of Tartu

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Contacts of UT units

Faculty of Arts and Humanities
Faculty phone: 
+372 737 5341
Faculty address: 
Jakobi 2, rooms 116–121, 51005 Tartu
  • Dean's Office
    Faculty phone: 
    + 372 737 5341
    Faculty address: 
    Jakobi 2, rooms 116–121, 51005 Tartu
  • Institute of History and Archaeology
    Faculty phone: 
    +372 737 5651
    Faculty address: 
    Jakobi 2, 51005 Tartu
  • Institute of Estonian and General Linguistics
    Faculty phone: 
    +372 737 5221
    Faculty address: 
    Jakobi 2, 51005 Tartu
  • Institute of Philosophy and Semiotics
    Faculty phone: 
    +372 737 5314
    Faculty address: 
    Jakobi 2, rooms 309–352, 51005 Tartu
  • Institute of Cultural Research
    Faculty phone: 
    (+372) 737 5223
    Faculty address: 
    Ülikooli 16, 51003 Tartu
  • School of Theology and Religious Studies
    Faculty phone: 
    +372 737 5301
    Faculty address: 
    Ülikooli 18–310, 50090 Tartu
  • College of Foreign Languages and Cultures
    Faculty address: 
    Lossi 3, 51003 Tartu
  • Viljandi Culture Academy
    Faculty phone: 
    +372 435 5232
    Faculty address: 
    Posti 1, 71004 Viljandi
Faculty of Social Sciences
Faculty phone: 
+372 737 5957
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Lossi 36, 51003 Tartu
  • Dean's Office
    Faculty phone: 
    + 372 737 5900
    Faculty address: 
    Lossi 36, 51003 Tartu
  • Institute of Education
    Faculty phone: 
    +372 737 6440
    Faculty address: 
    Salme 1a–29, 50103 Tartu
  • Johan Skytte Institute of Political Studies
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    +372 737 5582
    Faculty address: 
    Lossi 36–301, 51003 Tartu
  • School of Economics and Business Administration
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    Faculty address: 
    Narva mnt 18, 51009 Tartu
  • Institute of Psychology
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    Näituse 2, 50409 Tartu
  • School of Law
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  • Institute of Social Studies
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Faculty of Medicine
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  • Dean's Office
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    Ravila 19, 50411 Tartu
  • Institute of Biomedicine and Translational Medicine
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    Biomeedikum, Ravila 19, 50411 Tartu
  • Institute of Pharmacy
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  • Institute of Dentistry
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  • Institute of Clinical Medicine
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    L. Puusepa 8, 50406 Tartu
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    Faculty address: 
    Ravila 19, 50411 Tartu
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    Faculty phone: 
    +372 737 5360
    Faculty address: 
    Jakobi 5–205, 51005 Tartu
Faculty of Science and Technology
Faculty phone: 
+372 737 5820
Faculty address: 
Vanemuise 46–208, 51014 Tartu
  • Dean's Office
    Faculty phone: 
    +372 737 5820
    Faculty address: 
    Vanemuise 46–208, 51005 Tartu
  • Institute of Computer Science
    Faculty phone: 
    +372 737 5445
    Faculty address: 
    Narva mnt 18, 51009 Tartu
  • Estonian Marine Institute
    Faculty phone: 
    +372 671 8902
    Faculty address: 
    Mäealuse 14, 12618 Tallinn
  • Institute of Physics
    Faculty address: 
    W. Ostwaldi 1, 50411 Tartu
  • Institute of Chemistry
    Faculty phone: 
    +372 737 5261
    Faculty address: 
    Ravila 14a, 50411 Tartu
  • Institute of Mathematics and Statistics
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    +372 737 5860
    Faculty address: 
    Narva mnt 18, 51009 Tartu
  • Institute of Molecular and Cell Biology
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    Riia 23, 23b–134, 51010 Tartu
  • Tartu Observatory
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    Observatooriumi 1, Tõravere, 61602 Tartumaa
  • Institute of Technology
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    Faculty address: 
    Nooruse 1, 50411 Tartu
  • Institute of Ecology and Earth Sciences
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    Faculty address: 
    Vanemuise 46, 51003 Tartu
Institutions
  • Library
    Faculty phone: 
    +372 737 5702
    Faculty address: 
    W. Struve 1, 50091 Tartu
  • Youth Academy
    Faculty phone: 
    +372 737 5581
    Faculty address: 
    Uppsala 10, 51003 Tartu
  • Institute of Genomics
    Faculty phone: 
    +372 737 4000
    Faculty address: 
    Riia 23b, 51010 Tartu
  • Museum
    Faculty phone: 
    +372 737 5674
    Faculty address: 
    Lossi 25, 51003 Tartu
  • University of Tartu Natural History Museum and Botanical Garden
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    +372 737 6076
    Faculty address: 
    Vanemuise 46, 51003 Tartu
Support Units
  • Administrative Office
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    Faculty address: 
    Ülikooli 18a, 51005 Tartu
  • Centre for Entrepreneurship and Innovation
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    +372 737 6339
    Faculty address: 
    Lossi 3, III floor, 51003 Tartu
  • University Office in Tallinn
    Faculty phone: 
    +372 737 6600
    Faculty address: 
    Teatri väljak 3, 10143 Tallinn
  • Estates Office
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    +372 737 5137
    Faculty address: 
    Ülikooli 18a, 51005 Tartu
  • Finance Office
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    +372 737 5125
    Faculty address: 
    Jakobi 4, 51005 Tartu
  • Grant Office
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    +372 737 6215
    Faculty address: 
    Lossi 3, III floor, 51003 Tartu
  • Information Technology Office
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    Ülikooli 18a, 51005 Tartu
  • Human Resources Office
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    Ülikooli 18, rooms 302 and 304, 50090 Tartu
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  • Marketing and Communication Office
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    Faculty address: 
    Ülikooli 18, rooms 102, 104, 209, 210, 50090 Tartu
  • Office of Academic Affairs
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    Faculty address: 
    Ülikooli 18, 50090 Tartu
  • Procurement Office
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    Faculty address: 
    Ülikooli 18a, 51005 Tartu
  • Rector's Strategy Office
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    Faculty address: 
    Ülikooli 18, 50090 Tartu
  • Student Council
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    Ülikooli 18b, 51005 Tartu
  • University of Tartu Press
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    +372 737 5945
    Faculty address: 
    W. Struve 1, 50091 Tartu
Other Units
  • University of Tartu Academic Sports Club
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    +372 737 5371
    Faculty address: 
    Ujula 4, 51008 Tartu
  • Tartu Student Village
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    +372 740 9959
    Faculty address: 
    Narva mnt 25, 51013 Tartu
  • Tartu Students’ Club
    Faculty phone: 
    +372 730 2400
    Faculty address: 
    Kalevi 24, 51010 Tartu
  • Tartu University Hospital
    Faculty address: 
    L. Puusepa 1a, 50406 Tartu
  • University of Tartu Foundation
    Faculty phone: 
    +372 737 5852
    Faculty address: 
    Ülikooli 18, 50090 Tartu
  • View all other units

Margus Leppik "Substrate specificity of the multisite specific pseudouridine synthase RluD"

Date: 
29.08.2013 - 14:00 to 16:00

29. augustil 2013. aastal kell 14.00 kaitseb Margus Leppik will defence his doctoral thesis "Substrate specificity of the multisite specific pseudouridine synthase RluD" on the 29 August at 14 pm in the Institute of Molecular and Cell Biology of the University of Tartu (Riia 23B-105) in the specialty of Molecular Biology.

Supervisors:
Prof Jaanus Remme, Institute of Molecular and Cell Biology of the University of Tartu
Senior Research Fellow Aivar Liiv, Institute of Molecular and Cell Biology of the University of Tartu

Opponent:
Senior Lecturer Petr V. Sergiev, Faculty of Chemistry in the University of Moscow (Russian Federation)

Summary:
One of the most important processes in every cell lifecycle is protein synthesis performed by ribosomes. Ribosomes are composed of two unequal subunits (large and small subunit). Small subunit is responsible for following the genetic code and large subunit is responsible for synthesis of proteins. Ribosome consists of ribosomal RNA (rRNA) and r-proteins. Structure of rRNA is very conserved and it carries the catalytic role in protein synthesis. rRNA contains many modified nucleotides which are also highly conserved. Although modified nucleotides are known for a long time, there is little information about their function in rRNA. The most common modifications in rRNA are pseudouridines which are synthesized by proteins called pseudouridine synthases. E.coli RluD is pseudouridine synthase that synthesizes three highly conserved pseudouridines located in a very important structural element of the ribosome, helix 69 (H69). Little is known about how RluD recognizes its substrate nucleotides, or why these modifications are needed. We used genetic manipulations to introduce site specific point mutations into rRNA and RluD genes in order to determine the effect of the mutations on the occurrence of RluD directed pseudouridines. Our results show that RluD is specific to positions 1911, 1915, and 1917 in fully assembled ribosomes. However, RluD exhibits loosened specificity on protein free rRNA. Our results suggest that RluD exhibits a two-step binding to its substrate. The S4-like domain of RluD is responsible for initial binding that guides the catalytic domain to the substrate. We found that the catalytic domain of the RluD probably contacts nucleotide at position 1916 of the 23S rRNA to achieve its specificity. We also found that all RluD directed pseudouridines occur concurrently and independent of each other to the rRNA. Our results help to better understand the mechanism of ribosomal biogenesis in cells.

Organizer: 
Location: 
Riia 23B-105
Event category: