Marje Kasari "Bisubstrate luminescent probes, optical sensors and affinity adsorbents for measurement of active protein kinases in biological samples"

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View all other units

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Date:

16.03.2012 - 14:00 to 16:00

16. märtsil 2012 a. kell 14 kaitseb Marje Kasari Tartu Ülikooli loodus- ja tehnoloogiateaduskonnas (TÜ Chemicum aud. 1021, Tartu, Ravila 14A) doktoritööd "Bisubstrate luminescent probes, optical sensors and affinity adsorbents for measurement of active protein kinases in biological samples" (Bisubstraatsed luminestsentssondid, optilised sensorid ja afiinsusadsorbendid aktiivsete kinaaside määramiseks bioloogilistes proovides) bioorgaanilise keemia erialal.

Juhendaja: dr. Asko Uri
Oponent: prof. Tero Soukka

Proteiinkinaasid osalevad paljudes rakusisestes signaaliülekanderadades. Kõrvalekalded proteiinkinaaside aktiivsuse normaalsest tasemest võivad viia mitmete raskete haiguste tekkeni. Teadmine, et protiinkinaaside aktiivsust on võimalik reguleerida madalmolekulaarsete ühendite abil, on muutnud proteiinkinaasid oluliseks ravimiarenduse sihtmärgiks, eriti vähiravimite väljatöötamisel. Haiguste tekkel oluliste kinaaside identifitseerimisel on vajalikud analüüsimeetodid, mis võimaldavad tuvastada proteiinkiniaaside aktiivsuse muutust patsientide koeproovides. Teisalt on kinaaside kiired ja odavad analüüsimeetodid vajalikud haiguste diagnoosimiseks nende varajases staadiumis.
Käesoleva doktoritöö eesmärgiks oli meetodite arendamine aktiivsete proteiinkinaaside hulga määramiseks koeproovides. ARC-tüüpi bisubstraatsete inhibiitorite immobiliseerimisel saadi afiinsusadsorbendid ja sensorpinnad, mis võimaldasid proteiinkinaaside kontsentreerimist bioloogilistest proovidest ning nende kinaaside iseloomustamist pinna plasmonresonantsspektoskoopiat kasutades. Järgnevalt töötati välja üheetapiline meetod proteiinkinaaside määramiseks otse koeproovides. Töö käigus avastati, et mõnede ARC-tüüpi ühendite (ARC-Lum) kompleksid proteiinkinaasidega omavad varemkirjeldamata optilisi omadusi. Nende komplekside ergastamisel kiirgusega lähis-UV alas emiteerivad nad punases spektriosas valgust, mille sumbumise poolestusaeg on vahemikus 14-170 mikrosekundit, 10000 korda pikem kui tavalistel fluorestsentsvärvidel. Töötati välja ülitundlik ARC-Lum-sondide kasutamisel põhinev meetod vähi biomarkeri, rakuvälise PKAc (ECPKA), määramiseks inimese vereplasmas. Väljatöötatud meetod on üheetapiline, automatiseeritav, odav, teostatav levinud aparatuuri kasutades ning võimaldab mõõta pikomolaarseid ECPKA kontsentratsioone eeltöötlemata plasmaproovides.

Location:

Ravila 14A-1021

URL:

hdl.handle.net/10062/24148

Event category:

Defence

M	T	W	T	F	S	S
	1 Exhibition dedicated to the Estonian national university “Estonia of Ideals. 100 Years of Quests” The Exhibition "The enchanting art of stone cutting. Morgenstern 250" Estonia's National University 100: Photo exhibition “Put on Your Thinking Cap! The Hundred Faces of the University of Tartu” Aarne Maasik’s Photos of Tartu Cathedral	2 Gaygysyz Ashyrov „Essays on firm-level corruption” Exhibition dedicated to the Estonian national university “Estonia of Ideals. 100 Years of Quests” The Exhibition "The enchanting art of stone cutting. Morgenstern 250" Estonia's National University 100: Photo exhibition “Put on Your Thinking Cap! The Hundred Faces of the University of Tartu” Aarne Maasik’s Photos of Tartu Cathedral	3 Exhibition dedicated to the Estonian national university “Estonia of Ideals. 100 Years of Quests” The Exhibition "The enchanting art of stone cutting. Morgenstern 250" Estonia's National University 100: Photo exhibition “Put on Your Thinking Cap! The Hundred Faces of the University of Tartu” Aarne Maasik’s Photos of Tartu Cathedral	4 Kärt Ukkivi “Mutagenic effect of transcription and transcription-coupled repair factors in Pseudomonas putida” Ida Rahu „Bromine formation in inorganic bromide/nitrate mixtures and its application for oxidative aromatic bromination” Exhibition dedicated to the Estonian national university “Estonia of Ideals. 100 Years of Quests” The Exhibition "The enchanting art of stone cutting. Morgenstern 250" Estonia's National University 100: Photo exhibition “Put on Your Thinking Cap! The Hundred Faces of the University of Tartu” Aarne Maasik’s Photos of Tartu Cathedral	5 Exhibition dedicated to the Estonian national university “Estonia of Ideals. 100 Years of Quests” The Exhibition "The enchanting art of stone cutting. Morgenstern 250" Estonia's National University 100: Photo exhibition “Put on Your Thinking Cap! The Hundred Faces of the University of Tartu” Aarne Maasik’s Photos of Tartu Cathedral	6 Exhibition dedicated to the Estonian national university “Estonia of Ideals. 100 Years of Quests” The Exhibition "The enchanting art of stone cutting. Morgenstern 250" Estonia's National University 100: Photo exhibition “Put on Your Thinking Cap! The Hundred Faces of the University of Tartu” Aarne Maasik’s Photos of Tartu Cathedral
7 The inaugural lecture by Gholamreza Anbarjafari, UT Professor of of Computer Vision The Exhibition "The enchanting art of stone cutting. Morgenstern 250" Estonia's National University 100: Photo exhibition “Put on Your Thinking Cap! The Hundred Faces of the University of Tartu” Aarne Maasik’s Photos of Tartu Cathedral	8 The Exhibition "The enchanting art of stone cutting. Morgenstern 250" Estonia's National University 100: Photo exhibition “Put on Your Thinking Cap! The Hundred Faces of the University of Tartu” Aarne Maasik’s Photos of Tartu Cathedral	9 Kadi Luht-Kallas „Risk-taking behaviour: Relationship with personality and markers of heritability, and an intervention to prevent unintentional injury“ The Exhibition "The enchanting art of stone cutting. Morgenstern 250" Estonia's National University 100: Photo exhibition “Put on Your Thinking Cap! The Hundred Faces of the University of Tartu” Aarne Maasik’s Photos of Tartu Cathedral	10 Larisa Ivanova “Design of active compounds against neurodegenerative diseases” The Exhibition "The enchanting art of stone cutting. Morgenstern 250" Estonia's National University 100: Photo exhibition “Put on Your Thinking Cap! The Hundred Faces of the University of Tartu” Aarne Maasik’s Photos of Tartu Cathedral	11 The Exhibition "The enchanting art of stone cutting. Morgenstern 250" Estonia's National University 100: Photo exhibition “Put on Your Thinking Cap! The Hundred Faces of the University of Tartu” Aarne Maasik’s Photos of Tartu Cathedral	12 The Exhibition "The enchanting art of stone cutting. Morgenstern 250" Estonia's National University 100: Photo exhibition “Put on Your Thinking Cap! The Hundred Faces of the University of Tartu” Aarne Maasik’s Photos of Tartu Cathedral	13 The Exhibition "The enchanting art of stone cutting. Morgenstern 250" Estonia's National University 100: Photo exhibition “Put on Your Thinking Cap! The Hundred Faces of the University of Tartu” Aarne Maasik’s Photos of Tartu Cathedral
14 The Exhibition "The enchanting art of stone cutting. Morgenstern 250" Estonia's National University 100: Photo exhibition “Put on Your Thinking Cap! The Hundred Faces of the University of Tartu” Aarne Maasik’s Photos of Tartu Cathedral	15 Matti Vainio's exhibition "Findings \| Leiud" The Exhibition "The enchanting art of stone cutting. Morgenstern 250" Estonia's National University 100: Photo exhibition “Put on Your Thinking Cap! The Hundred Faces of the University of Tartu” Aarne Maasik’s Photos of Tartu Cathedral	16 Matti Vainio's exhibition "Findings \| Leiud" The Exhibition "The enchanting art of stone cutting. Morgenstern 250" Estonia's National University 100: Photo exhibition “Put on Your Thinking Cap! The Hundred Faces of the University of Tartu” Aarne Maasik’s Photos of Tartu Cathedral	17 Matti Vainio's exhibition "Findings \| Leiud" The Exhibition "The enchanting art of stone cutting. Morgenstern 250" Estonia's National University 100: Photo exhibition “Put on Your Thinking Cap! The Hundred Faces of the University of Tartu” Aarne Maasik’s Photos of Tartu Cathedral	18 Sten Saar "Epidemiology of severe injuries in Estonia" Kadi Tilk „Signals and responses of ColRS two-component system in Pseudomonas putida“ Matti Vainio's exhibition "Findings \| Leiud" The Exhibition "The enchanting art of stone cutting. Morgenstern 250" Estonia's National University 100: Photo exhibition “Put on Your Thinking Cap! The Hundred Faces of the University of Tartu” Aarne Maasik’s Photos of Tartu Cathedral	19 Matti Vainio's exhibition "Findings \| Leiud" The Exhibition "The enchanting art of stone cutting. Morgenstern 250" Estonia's National University 100: Photo exhibition “Put on Your Thinking Cap! The Hundred Faces of the University of Tartu” Aarne Maasik’s Photos of Tartu Cathedral	20 Matti Vainio's exhibition "Findings \| Leiud" The Exhibition "The enchanting art of stone cutting. Morgenstern 250" Estonia's National University 100: Photo exhibition “Put on Your Thinking Cap! The Hundred Faces of the University of Tartu” Aarne Maasik’s Photos of Tartu Cathedral
21 Matti Vainio's exhibition "Findings \| Leiud" The Exhibition "The enchanting art of stone cutting. Morgenstern 250" Estonia's National University 100: Photo exhibition “Put on Your Thinking Cap! The Hundred Faces of the University of Tartu” Aarne Maasik’s Photos of Tartu Cathedral	22 Ilya Kuzovkin „Understanding Information Processing in Human Brain by Interpreting Machine Learning Models“ Matti Vainio's exhibition "Findings \| Leiud" The Exhibition "The enchanting art of stone cutting. Morgenstern 250" Estonia's National University 100: Photo exhibition “Put on Your Thinking Cap! The Hundred Faces of the University of Tartu” Aarne Maasik’s Photos of Tartu Cathedral	23 Matti Vainio's exhibition "Findings \| Leiud" The Exhibition "The enchanting art of stone cutting. Morgenstern 250" Estonia's National University 100: Photo exhibition “Put on Your Thinking Cap! The Hundred Faces of the University of Tartu” Aarne Maasik’s Photos of Tartu Cathedral	24 UT Memorial Day Matti Vainio's exhibition "Findings \| Leiud" The Exhibition "The enchanting art of stone cutting. Morgenstern 250" Estonia's National University 100: Photo exhibition “Put on Your Thinking Cap! The Hundred Faces of the University of Tartu” Aarne Maasik’s Photos of Tartu Cathedral	25 Orlenys López Pintado “Collaborative Business Process Execution on the Blockchain: The Caterpillar System“ Mari Urb "DNA methylation in the predisposition, expression and abstinence of cocaine addiction" Researchers' Night Festival: Toome science-themed adventure town special Matti Vainio's exhibition "Findings \| Leiud" The Exhibition "The enchanting art of stone cutting. Morgenstern 250" Estonia's National University 100: Photo exhibition “Put on Your Thinking Cap! The Hundred Faces of the University of Tartu” Aarne Maasik’s Photos of Tartu Cathedral	26 Researchers' Night Festival and Day of Countryside Living at Tartu Observatory Matti Vainio's exhibition "Findings \| Leiud" The Exhibition "The enchanting art of stone cutting. Morgenstern 250" Estonia's National University 100: Photo exhibition “Put on Your Thinking Cap! The Hundred Faces of the University of Tartu” Aarne Maasik’s Photos of Tartu Cathedral	27 Matti Vainio's exhibition "Findings \| Leiud" The Exhibition "The enchanting art of stone cutting. Morgenstern 250" Estonia's National University 100: Photo exhibition “Put on Your Thinking Cap! The Hundred Faces of the University of Tartu” Aarne Maasik’s Photos of Tartu Cathedral
28 Matti Vainio's exhibition "Findings \| Leiud" The Exhibition "The enchanting art of stone cutting. Morgenstern 250" Estonia's National University 100: Photo exhibition “Put on Your Thinking Cap! The Hundred Faces of the University of Tartu” Aarne Maasik’s Photos of Tartu Cathedral	29 Matti Vainio's exhibition "Findings \| Leiud" The Exhibition "The enchanting art of stone cutting. Morgenstern 250" Estonia's National University 100: Photo exhibition “Put on Your Thinking Cap! The Hundred Faces of the University of Tartu” Aarne Maasik’s Photos of Tartu Cathedral	30 Concert: smooth harmonies Matti Vainio's exhibition "Findings \| Leiud" The Exhibition "The enchanting art of stone cutting. Morgenstern 250" Estonia's National University 100: Photo exhibition “Put on Your Thinking Cap! The Hundred Faces of the University of Tartu” Aarne Maasik’s Photos of Tartu Cathedral

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Marje Kasari "Bisubstrate luminescent probes, optical sensors and affinity adsorbents for measurement of active protein kinases in biological samples"

September